Journal: Journal of Translational Medicine
Article Title: A validated cellular biobank for β-thalassemia
doi: 10.1186/s12967-016-1016-4
Figure Lengend Snippet: a Representative experiment showing the variation of expression of the hematopoietic stem cell marker CD117, adhesion molecule marker CD44, beta1-integrin surface marker CD29, and erythroid differentiation marker CD235 (GPA) in undifferentiated cells (day 0) and in cells at two progressive stages of erythroid maturation (day 4 and 8). b – i Quantitative data showing the % of cells expressing the indicated markers ( b – e ) and the mean fluorescence values (MF) ( f – i ) after FACS analyses using antibodies recognizing CD117 ( b , f ), CD44 ( c , g ), CD29 ( d , h ) and GPA ( e , i ). The data represent the average ±SD obtained in seven independent experiments using vials obtained from 7 different β-thalassemia patients
Article Snippet: Five ml of expansion medium contains: StemSpan Serum-Free Medium Expansion (Voden, Vancouver, Canada), 50 μl of StemSpan® CC100 Cytokine Cocktail for Expansion of Human Hematopoietic Cells Stem Cell Technologies (Voden, Vancouver, Canada), 2 U/mL erythropoietin (EPO, Tebu-bio, Magenta, Milan, Italy), 10 −6 M dexamethasone (Dexamethasone 21-phosphate disodium salt, Sigma-Aldrich, Saint Louis, Missouri, USA), 50 μl of a 100× Pennicilin/Streptomycin solution (Lonza, Verviers, Belgium).
Techniques: Expressing, Marker, Fluorescence